Exhibit 25.1



securities and exchange commission

Washington, D.C. 20549





Statement of Eligibility Under

The Trust Indenture Act of 1939 of a

Corporation Designated to Act as Trustee

Check if an Application to Determine Eligibility of

a Trustee Pursuant to Section 305(b)(2)




(Exact name of Trustee as specified in its charter)



I.R.S. Employer Identification No.


800 Nicollet Mall

Minneapolis, Minnesota



(Address of principal executive offices)

(Zip Code)


Raymond S. Haverstock

U.S. Bank National Association

60 Livingston Avenue

St. Paul, MN 55107

(651) 466-6299

(Name, address and telephone number of agent for service)


Calyxt, Inc.

                                                       (Issuer with respect to the Securities)



(State or other jurisdiction of incorporation or organization)

(I.R.S. Employer Identification No.)




2800 Mount Ridge Road

Roseville, MN



(Address of Principal Executive Offices)

(Zip Code)


Debt Securities

(Title of the Indenture Securities)







Item 1.      GENERAL INFORMATION.  Furnish the following information as to the Trustee.




Name and address of each examining or supervising authority to which it is subject.

Comptroller of the Currency

Washington, D.C.


b)Whether it is authorized to exercise corporate trust powers.



Item 2.     AFFILIATIONS WITH OBLIGOR. If the obligor is an affiliate of the Trustee, describe each such affiliation.



Items 3-15

Items 3-15 are not applicable because to the best of the Trustee's knowledge, the obligor is not in default under any Indenture for which the Trustee acts as Trustee.


Item 16.     LIST OF EXHIBITS: List below all exhibits filed as a part of this statement of eligibility and qualification.


1.   A copy of the Articles of Association of the Trustee.*


2.   A copy of the certificate of authority of the Trustee to commence business, attached as Exhibit 2.


3.A copy of the certificate of authority of the Trustee to exercise corporate trust powers, attached as Exhibit 3.


4.A copy of the existing bylaws of the Trustee.**


5.A copy of each Indenture referred to in Item 4.  Not applicable.


6.The consent of the Trustee required by Section 321(b) of the Trust Indenture Act of 1939, attached as Exhibit 6.


7.Report of Condition of the Trustee as of March 31, 2019 published pursuant to law or the requirements of its supervising or examining authority, attached as Exhibit 7.


* Incorporated by reference to Exhibit 25.1 to Amendment No. 2 to registration statement on S-4, Registration Number 333-128217 filed on November 15, 2005.


** Incorporated by reference to Exhibit 25.1 to registration statement on form S-3ASR,  Registration Number 333-199863 filed on November 5, 2014.







Pursuant to the requirements of the Trust Indenture Act of 1939, as amended, the Trustee, U.S. BANK NATIONAL ASSOCIATION, a national banking association organized and existing under the laws of the United States of America, has duly caused this statement of eligibility and qualification to be signed on its behalf by the undersigned, thereunto duly authorized, all in the City of St. Paul, State of Minnesota on the 9th of August, 2019.


By:/s/ Raymond S. Haverstock________Raymond S. Haverstock

Vice President









































Exhibit 2








Exhibit 3







Exhibit 6





In accordance with Section 321(b) of the Trust Indenture Act of 1939, the undersigned, U.S. BANK NATIONAL ASSOCIATION hereby consents that reports of examination of the undersigned by Federal, State, Territorial or District authorities may be furnished by such authorities to the Securities and Exchange Commission upon its request therefor.



Dated: August 9,  2019



By:/s/ Raymond S. Haverstock_________Raymond S. Haverstock

Vice President







Exhibit 7


U.S. Bank National Association

Statement of Financial Condition

As of 3/31/2019






Cash and Balances Due From $ 18,011,992

           Depository Institutions


Federal Funds3,518,495

Loans & Lease Financing Receivables 286,352,008

Fixed Assets               5,289,051

Intangible Assets12,998,717

Other Assets   27,522,814

Total Assets    $467,322,170




Fed Funds1,408,144

Treasury Demand Notes0

Trading Liabilities  565,646

Other Borrowed Money37,549,120


Subordinated Notes and Debentures3,800,000

Other Liabilities15,767,654

Total Liabilities$418,242,521



      Common and Preferred Stock18,200

Surplus 14,266,915

Undivided Profits33,995,325

Minority Interest in Subsidiaries799,209

Total Equity Capital$49,079,649


Total Liabilities and Equity Capital$467,322,170